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First published online on July 14, 2008
Chest, doi:10.1378/chest.07-1100
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Expression of ILT4 Inhibitory Receptor in Human Non-Small Cell Lung Cancer

Yuping Sun, PhD; Jie Liu, MD; Ping Gao; Yunshan Wang, PhD and Chuanyong Liu, MD

Jinan Central Hospital, Shandong University, Jinan, Shandong 250013, People's Republic of China

sunyupingsyp{at}hotmail.com

sdjnwys{at}163.com

Abstract

BackgroundHuman Ig-like transcript 4 (ILT-4) is a member of the inhibitory receptor family for immune function. Little is known about the expression levels of ILT-4 in tumor cells.

MethodsWe have studied the expression levels of ILT-4 both in vitro in cancer cell lines and in vivo tumor tissues from 70 patients with non-small cell lung cancer (NSCLC) by RT-PCR, FACS and immunohistochemical analyses.

ResultsThree cancer cell lines (H1299, A549 and U1810) express ILT4 mRNA and only two (H1299 and A549) express ILT4 protein on the cell surface. About 37.1% of 70 tumor tissue samples express ILT-4, which is localized in both cell membrane and the cytoplasm. In addition, tumor cells, stromal and plasma cells also express ILT-4. The number of T filtrating lymphoid cells (TILs) in the tumor tissues that express B7-H3 was much lower than those who didn't. But there is no significant correlation between ILT-4 expression and disease progression including nodal metastasis.

ConclusionsThese findings suggest that ILT4 is frequently expressed in both tumor and stromal cells of NSCLC and it might play an important role in regulation of the host immune system.

Key Words: ILT-4 • immunohistochemistry • non-small-cell lung cancer • infiltrating lymphoid cells (TILs)






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